Preface
The use of bioreactors in cell biology is becoming more commonplace as attempts are made
to scale up production of various types of cells for regenerative medicine and pharmaceutical
purposes. Given the rapidity with which bioreactor technologies are advancing and being
applied, I have attempted to bring together some of the latest developments and protocols
in this second edition on this topic. The protocols in this volume are all new protocols and
reflect novel technology development and potential uses that I hope will benefit both
established investigators and newcomers to the field.
Once again, the protocols gathered here are faithful to the mission statement of the
Methods in Molecular Biology series: They are well-established and described in an easy-to-
follow, step-by-step fashion so as to be valuable for not only experts but also novices in the
stem cell field. That goal is achieved because of the generosity of the contributors who have
carefully described their protocols in this volume, and we are very grateful for their efforts.
Our thanks as well go to Dr. John Walker, the Editor-in-Chief of the Methods in
Molecular Biology series, for giving us the opportunity to create and now update this volume
and for supporting us along the way.
We are also grateful to Patrick Marton, the Executive Editor of Methods in Molecular
Biology and the Springer Protocols collection, for his continuous support from idea to
completion of this volume.
A special thank you goes to Anna Rakovsky, Assistant Editor for Methods in Molecular
Biology, for continuous support from the beginning to the end of this project.
We would also like to thank David C. Casey, the Editor of Methods in Molecular Biology,
for his outstanding editorial work during the production of this volume.
Finally, we would like to thank Daniel Ignatius Jagadisan, Sarumathi Hemachandrane,
Anand Ventakachalam, and the rest of the production crew for their work in putting
together an outstanding volume.
Ottawa, ON, Canada
Kursad Turksen
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